Journal: International Journal of Nanomedicine
Article Title: Gene-silencing effects of anti-survivin siRNA delivered by RGDV-functionalized nanodiamond carrier in the breast carcinoma cell line MCF-7
doi: 10.2147/IJN.S117611
Figure Lengend Snippet: Synthesis of NDCONH(CH 2 ) 2 NH-VDGR/survivin-siRNA. Conditions in each step: (i) H 2 SO 4 /HNO 3 ; (ii) SOCl 2 ; (iii) NH 2 (CH 2 ) 2 NH-Boc/THF and HCl/EA; and (iv) EDC/Boc-Arg(Tos)-Gly-Asp(OMe)-Val-OH, NaOH/MeOH, and TFA/TfOH. Abbreviations: siRNA, small interfering RNA; THF, tetrahydrofuran; EA, ethyl alcohol; EDC, N1-((ethylimino)methylene)-N3,N3-dimethylpropane-1,3-diamine; TFA, trifluoroacetic acid; TfOH, trifluoromethanesulfonic acid; ND, nanodiamond.
Article Snippet: Boc-Arg(Tos)-OH, H-Gly-OBzl⋅HCl, Boc-Asp(OMe)-OH, H-Val-OBzl⋅HCl, dicyclohexylcarbodiimide, N1-((ethylimino) methylene)-N3,N3-dimethylpropane-1,3-diamine (EDC), and hydroxybenzotriazole (HOBt) were purchased from GL Biochem Ltd (Shanghai, People’s Republic of China).
Techniques: Small Interfering RNA